| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hart, Madeline J (19)
|
OHABLY |
2:24.47 |
2:30.81 |
6.34 |
| 2 |
Reid, McKenzie D (18)
|
OHKEY |
2:50.58 |
2:34.18 |
-16.40 |
| 3 |
Pressler, Kathleen N (16)
|
OHPCY |
|
2:36.07 |
- |
| 4 |
Johnson, Alexandra F (16)
|
OHABLY |
2:24.63 |
2:36.54 |
11.91 |
| 5 |
Slaney, Morgan A (17)
|
OHABLY |
2:26.47 |
2:39.64 |
13.17 |
| 6 |
DeCarlo, Maria E (16)
|
OHPCY |
|
3:02.38 |
- |
| 7 |
Knapek, Kaitlin N (16)
|
OHLAKY |
3:05.01 |
3:02.92 |
-2.09 |
| 8 |
Woods, Sophia (16)
|
OHLAKY |
3:32.30 |
3:12.35 |
-19.95 |
| 9 |
Eldridge, Yamileth L (15)
|
OHPCY |
3:18.71 |
3:22.79 |
4.08 |
| 10 |
Bodager, Paisley (15)
|
KYNLY |
|
4:11.77 |
- |
|
Tenhundfeld, Ava J (15)
|
OHKEY |
2:38.06 |
NS |
- |