| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Thomas P (16)
|
OHRCDB |
54.03 |
52.89 |
-1.14 |
| 2 |
Holmes, Jeremiah, Jr. J (16)
|
KYAAY |
55.20 |
53.45 |
-1.75 |
| 3 |
Herfel, Garrison F (18)
|
OHPCY |
51.84 |
56.87 |
5.03 |
| 4 |
Varga, Charlie (15)
|
INWCFY |
58.84 |
57.41 |
-1.43 |
| 5 |
Holmes, Calyx J (15)
|
KYAAY |
58.59 |
57.78 |
-0.81 |
| 6 |
Olmstead, Shaine M (18)
|
OHRCDB |
56.63 |
59.74 |
3.11 |
| 7 |
Chapman, Avery C (17)
|
OHRCDB |
1:01.50 |
1:03.04 |
1.54 |
| 8 |
Ryan, Connor C (16)
|
OHRCDB |
1:08.07 |
1:03.48 |
-4.59 |
| 9 |
Naehr, John (15)
|
KYBYB |
1:07.73 |
1:05.31 |
-2.42 |
| 10 |
Schierenbeck, Camden L (16)
|
OHRCDB |
1:09.11 |
1:05.44 |
-3.67 |
| 11 |
Goggin, Ethan (15)
|
INWCFY |
1:08.24 |
1:08.32 |
0.08 |
| 12 |
Akers/Perry, Dakota R (15)
|
OHCY |
1:10.54 |
1:09.43 |
-1.11 |
| 13 |
Hoffman, Phillip A (15)
|
OHRCDB |
1:19.70 |
1:09.64 |
-10.06 |
| 14 |
Proctor, Mac (15)
|
KYBYB |
1:16.97 |
1:12.20 |
-4.77 |
| 15 |
Hebenstiel, Lars (15)
|
OHRCDB |
1:20.34 |
1:21.20 |
0.86 |
|
Cole, Marcus G (15)
|
OHRCDB |
1:01.64 |
NS |
- |