| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lambert, Laina R (12)
|
OHSPY |
41.13 |
40.75 |
-0.38 |
| 2 |
Yu, Grace * (12)
|
OHBASH |
|
41.23 |
- |
| 3 |
Derr, Lola T (12)
|
OHSPY |
39.67 |
41.37 |
1.70 |
| 4 |
Stephens, Gabriella E (12)
|
OHRCDB |
44.64 |
41.62 |
-3.02 |
| 5 |
Fink, Sophia M (11)
|
OHLAKY |
50.69 |
42.78 |
-7.91 |
| 6 |
Hayes, Charleigh B (12)
|
OHBASH |
|
43.06 |
- |
| 7 |
Spetz, Johanna R (12)
|
OHLAKY |
49.35 |
43.25 |
-6.10 |
| 8 |
Finn, Sarah E (12)
|
OHBASH |
51.94 |
43.45 |
-8.49 |
| 9 |
Mulvihill, Caroline C (12)
|
OHABLY |
|
43.49 |
- |
| 10 |
Sutphin, Audrina N (12)
|
OHABLY |
46.01 |
43.65 |
-2.36 |
| 11 |
Squibb, Jocelyn A (12)
|
OHLAKY |
50.57 |
43.84 |
-6.73 |
| 12 |
Preston, Evelyn C (12)
|
OHABLY |
50.73 |
44.45 |
-6.28 |
| 13 |
Irmscher, Layla R (12)
|
OHAMFY |
51.42 |
45.15 |
-6.27 |
| 14 |
Armstrong, Molly B (12)
|
OHABLY |
52.25 |
45.25 |
-7.00 |
| 15 |
Meyer, Audrey K (12)
|
OHAMFY |
|
45.43 |
- |
| 16 |
Bennett, Emily R (12)
|
OHSPY |
46.49 |
45.58 |
-0.91 |
| 17 |
Miller, Lydia E (12)
|
OHLAKY |
54.33 |
47.16 |
-7.17 |
| 18 |
Linnemann, Sarah M (12)
|
OHPCY |
|
47.18 |
- |
| 19 |
Smyth, Norah M (12)
|
OHBASH |
|
47.34 |
- |
| 20 |
Buot, Anna C (12)
|
OHABLY |
49.25 |
48.16 |
-1.09 |
| 21 |
Nguyen, Ai Nhi M (11)
|
OHABLY |
53.21 |
48.37 |
-4.84 |
| 22 |
Varvel, Olive A (11)
|
OHKEY |
|
49.23 |
- |
| 23 |
Fada, Natalie C (12)
|
OHLAKY |
57.41 |
49.37 |
-8.04 |
| 24 |
Auger, Amelia J (11)
|
OHPCY |
55.49 |
49.59 |
-5.90 |
| 25 |
Holtmeier, Katherine M (11)
|
OHLAKY |
|
49.67 |
- |
| 26 |
Cooley, Gayle R (12)
|
OHLAKY |
1:02.76 |
49.74 |
-13.02 |
| 27 |
Chaubey, Mahi P (11)
|
OHLAKY |
|
49.81 |
- |
| 28 |
Simpson, Addison J (12)
|
OHLAKY |
59.69 |
50.65 |
-9.04 |
| 29 |
Schroeder, Hannah R (11)
|
OHBASH |
57.16 |
50.75 |
-6.41 |
| 30 |
Wolfe, Sophialyn H (11)
|
OHRCDB |
1:03.73 |
51.29 |
-12.44 |
| 31 |
Huey, Evelyn M (11)
|
OHPCY |
58.06 |
52.11 |
-5.95 |
| 32 |
Ray, Madison E (12)
|
OHLAKY |
1:11.57 |
52.62 |
-18.95 |
| 33 |
Menne, Alexis R (11)
|
OHABLY |
1:01.48 |
54.83 |
-6.65 |
| 34 |
Murlin, Madeline M (12)
|
OHHHY |
59.45 |
55.47 |
-3.98 |
| 35 |
Liesenfeld, Livia H (12)
|
OHRCDB |
|
56.30 |
- |
| 36 |
Hill, Nevaeh J (11)
|
OHHHY |
|
56.45 |
- |
| 37 |
Woods, Samantha L (11)
|
OHLAKY |
1:09.69 |
57.87 |
-11.82 |
| 38 |
Hobbs, Catherine A (11)
|
OHPCY |
1:02.16 |
57.92 |
-4.24 |
| 39 |
Jackson, Margaret A (11)
|
OHGMVY |
|
1:13.52 |
- |
| 40 |
Jago, Brianna J (12)
|
OHPCY |
|
1:16.49 |
- |
|
Murphy, Rosemary C (11)
|
OHPCY |
59.14 |
NS |
- |
|
Lang, Charlotte M (11)
|
OHBASH |
|
NS |
- |