| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peck, Jonathan L (10)
|
OHGMVY |
39.25 |
39.51 |
0.26 |
| 2 |
Hernandez, Noah E (9)
|
OHCFY |
37.60 |
44.23 |
6.63 |
| 3 |
Van Schoyck, Grayden C (9)
|
OHCY |
41.89 |
44.81 |
2.92 |
| 4 |
Drohan, Breckin M (9)
|
OHCY |
49.74 |
47.20 |
-2.54 |
| 5 |
Lusk, J.J. J (10)
|
OHLAKY |
47.22 |
47.62 |
0.40 |
| 6 |
Hu, Jonathan C (10)
|
OHBASH |
46.01 |
48.25 |
2.24 |
| 7 |
Williams, Andrew S (10)
|
OHPCY |
47.49 |
48.50 |
1.01 |
| 8 |
Perica, Arlo H (9)
|
OHPCY |
55.12 |
50.45 |
-4.67 |
| 9 |
Irwin, Wyatt G (10)
|
OHABLY |
47.95 |
51.54 |
3.59 |
| 10 |
Howard, William J (10)
|
OHSPY |
53.20 |
51.98 |
-1.22 |
| 11 |
Wang, Andrew S (10)
|
OHCFY |
55.62 |
52.55 |
-3.07 |
| 12 |
Braun, William P (10)
|
OHPCY |
52.52 |
52.56 |
0.04 |
| 13 |
Childers, Joey L (10)
|
OHRCDB |
53.03 |
53.09 |
0.06 |
| 14 |
Wilson, Finnigan M (10)
|
OHSPY |
53.41 |
53.41 |
0.00 |
| 15 |
Preston, Elliott C (10)
|
OHABLY |
56.77 |
55.68 |
-1.09 |
| 16 |
Gee, Gavin M (10)
|
OHCY |
51.82 |
56.85 |
5.03 |
| 17 |
Gabbard, Paxton C (10)
|
OHPCY |
56.86 |
57.63 |
0.77 |
| 18 |
Yu, Alex L (9)
|
OHBASH |
1:03.76 |
1:00.47 |
-3.29 |
| 19 |
Schmid, Matthew M (9)
|
OHBASH |
55.35 |
1:00.58 |
5.23 |
| 20 |
Kemphues, Samuel B (10)
|
OHABLY |
1:09.74 |
1:03.51 |
-6.23 |
| 21 |
Lickliter, Harrison D (9)
|
OHPCY |
56.56 |
1:03.55 |
6.99 |
| 22 |
Janybek, Emir J (9)
|
OHBASH |
3:00.00 |
1:06.95 |
-113.05 |
| 23 |
Vijayakumar, Vishwa G (10)
|
OHLAKY |
1:11.29 |
1:08.88 |
-2.41 |
| 24 |
Reyes, Gabriel J (10)
|
OHCY |
59.75 |
1:09.37 |
9.62 |
| 25 |
Fox, Jay B (9)
|
OHPCY |
1:09.81 |
1:11.09 |
1.28 |
| 26 |
Norris, Rocco J (9)
|
OHABLY |
54.62 |
1:12.96 |
18.34 |
| 27 |
Murlin, Zachary D (9)
|
OHHHY |
1:29.67 |
1:19.19 |
-10.48 |
| 28 |
Joshi, Neil H (9)
|
OHLAKY |
1:21.39 |
1:20.62 |
-0.77 |
|
Smith, Henry J (9)
|
OHABLY |
1:26.13 |
DQ |
- |