| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Furuta, Roman R (16)
|
OHRCDB |
22.71 |
22.71 |
0.00 |
| 2 |
Brown, Jack H (15)
|
OHRCDB |
23.32 |
22.87 |
-0.45 |
| 3 |
Coop, Parker B (16)
|
OHRCDB |
24.18 |
23.71 |
-0.47 |
| 4 |
Stephens, Zachary D (15)
|
OHRCDB |
23.82 |
24.22 |
0.40 |
| 5 |
Bame, Joshua C (17)
|
KYBYB |
23.17 |
24.55 |
1.38 |
| 6 |
Dattilio, Mark L (16)
|
KYBYB |
23.83 |
24.57 |
0.74 |
| 7 |
Greinke, Elijah R (15)
|
KYBYB |
24.92 |
24.85 |
-0.07 |
| 8 |
Schwartz, Jordan P (16)
|
OHAMFY |
|
25.38 |
- |
| 9 |
Morgan IV, Jesse D (18)
|
OHHHY |
25.25 |
25.41 |
0.16 |
| 10 |
Richter, Camden J (17)
|
OHMCY |
23.97 |
25.57 |
1.60 |
| 11 |
Hafner, Isaac C (15)
|
OHGMVY |
24.86 |
25.77 |
0.91 |
| 12 |
Cagle, Samuel P (15)
|
OHPCY |
25.97 |
25.84 |
-0.13 |
| 13 |
Pache, Daniel (18)
|
INYOGI |
24.71 |
26.04 |
1.33 |
| 14 |
Christenson, Matthew D (16)
|
OHMCY |
25.22 |
26.50 |
1.28 |
| 15 |
Tullis, Noah C (15)
|
OHMCY |
25.10 |
28.09 |
2.99 |
| 16 |
Bellis, Hunter J (15)
|
KYBYB |
25.99 |
28.20 |
2.21 |
| 17 |
Davis, Jaxson G (15)
|
OHCFY |
|
28.22 |
- |
| 18 |
Simpson, Aaron B (15)
|
OHMCY |
27.68 |
28.31 |
0.63 |
| 19 |
Barker, Nathaniel L (15)
|
KYBYB |
29.25 |
29.31 |
0.06 |
| 20 |
Hardin, Mitchell (15)
|
INYOGI |
32.12 |
30.73 |
-1.39 |
|
Galbraith, Ruarigh P (15)
|
OHCFY |
25.65 |
NS |
- |
|
Parke, Braden M (15)
|
OHRCDB |
27.93 |
NS |
- |