| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhao, Jason J (14)
|
OHBASH |
2:06.27 |
2:00.85 |
-5.42 |
| 2 |
Brady, Noah S (14)
|
OHBASH |
2:15.87 |
2:11.41 |
-4.46 |
| 3 |
Brockman, Dominic R (14)
|
OHRCDB |
2:24.96 |
2:21.97 |
-2.99 |
| 4 |
Welty, Max J (13)
|
OHBASH |
2:30.75 |
2:22.64 |
-8.11 |
| 5 |
Gessner, Drew C (14)
|
OHMCY |
2:16.53 |
2:23.17 |
6.64 |
| 6 |
Fuller, Braden P (13)
|
OHLAKY |
2:25.66 |
2:23.87 |
-1.79 |
| 7 |
Naehr, William J (14)
|
KYBYB |
2:36.35 |
2:40.04 |
3.69 |
| 8 |
Gropp, James (13)
|
INYOGI |
2:39.56 |
2:40.47 |
0.91 |
| 9 |
Golden, Oliver W (13)
|
OHABLY |
2:49.23 |
2:42.78 |
-6.45 |
| 10 |
Smith, Samuel K (13)
|
OHKEY |
2:49.65 |
2:43.26 |
-6.39 |
| 11 |
Joshi, Jaison (13)
|
OHLAKY |
2:49.92 |
2:43.34 |
-6.58 |
| 12 |
Lambrinides, Jackson T (13)
|
OHPCY |
|
2:44.41 |
- |
| 13 |
Kozen, Benjamin K (14)
|
OHMCY |
|
2:54.40 |
- |
| 14 |
Day-Klaiber, Greysen J (13)
|
KYBYB |
|
3:00.21 |
- |
| 15 |
Peddicord, Jonathan A (14)
|
KYBYB |
|
3:04.48 |
- |
| 16 |
Phelps, Abram G (13)
|
KYBYB |
|
3:24.82 |
- |
|
Pressler, Charles W (13)
|
OHPCY |
2:57.28 |
DQ |
- |
|
Stakauskas, Andrew W (14)
|
OHPCY |
2:34.06 |
DQ |
- |