| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Marina C (10)
|
MAMSA |
35.45 |
32.16 |
-3.29 |
| 1 |
Watkins, Azlynn K (10)
|
MAUN-1 |
|
35.22 |
- |
| 1 |
Lochstet, Peregrine E (10)
|
MAUDAC |
36.80 |
36.01 |
-0.79 |
| 2 |
Shannon, Kimberly G (10)
|
MASUNY |
36.00 |
33.18 |
-2.82 |
| 2 |
Houck, Jordan R (10)
|
AMHLBG |
37.52 |
36.26 |
-1.26 |
| 2 |
Brandt, Madelyn R (9)
|
MAWAC |
50.99 |
36.72 |
-14.27 |
| 3 |
Vorobyeva, Arina I (9)
|
MAUDAC |
34.06 |
34.62 |
0.56 |
| 3 |
Dudkina, Elena V (10)
|
MACBST |
|
36.77 |
- |
| 3 |
Rhoge, Elana S (10)
|
MATHY |
36.34 |
37.51 |
1.17 |
| 4 |
Ernst, Carley A (9)
|
MADSC |
34.17 |
34.91 |
0.74 |
| 4 |
Ladig, Elin S (9)
|
MAWAC |
|
36.84 |
- |
| 5 |
McClain, Madelyn G (10)
|
AMNAC |
35.57 |
35.33 |
-0.24 |
| 5 |
Suko, Caroline E (10)
|
MACBST |
|
37.38 |
- |
| 5 |
Harberson, Sophie S (10)
|
MADSC |
|
37.38 |
- |
| 7 |
Kellner, Gabriella H (9)
|
MACBST |
|
38.26 |
- |
| 8 |
Majewski, Madeline J (9)
|
MACBST |
|
39.69 |
- |
| 9 |
Cohick, Kendall A (9)
|
MAWASC |
41.02 |
40.27 |
-0.75 |
| 10 |
Milhomme, Saylor (10)
|
MANLAC |
55.99 |
41.18 |
-14.81 |
| 11 |
Gilligan, Rena A (10)
|
AMWAAC |
45.71 |
41.35 |
-4.36 |
| 12 |
Hooper, Kaitlin A (10)
|
MANLAC |
41.43 |
42.15 |
0.72 |
| 13 |
Tallapragada, Harshitha T (9)
|
MAMSA |
46.41 |
42.56 |
-3.85 |
| 14 |
Doucette, Kate L (10)
|
MANLAC |
46.29 |
43.37 |
-2.92 |
| 15 |
Turner, Autumn A (10)
|
AMSASC |
41.50 |
44.99 |
3.49 |
| 16 |
Belair, Emily G (10)
|
MAUN-2 |
|
46.67 |
- |
| 17 |
Hartley, Sophia G (9)
|
MAMSA |
51.77 |
52.86 |
1.09 |
| 18 |
Collery, Brenna E (9)
|
AMSASC |
|
53.92 |
- |
| 19 |
Catlin, Eliza C (9)
|
AMWAAC |
49.02 |
1:00.92 |
11.90 |
|
Knopsnyder, Ava N (9)
|
MAUN-2 |
|
35.50 |
- |