| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bedford, Ainsley N (12)
|
NIWEBS |
40.96 |
39.24 |
-1.72 |
| 2 |
Keyes, Angelina T (12)
|
NIGRSC |
43.45 |
39.33 |
-4.12 |
| 3 |
Maynard, Chelsea A (11)
|
NISDSC |
42.73 |
41.37 |
-1.36 |
| 4 |
Evans, Mary A (12)
|
NIGRSC |
41.34 |
41.40 |
0.06 |
| 5 |
Skizim, Elena K (11)
|
NIUN-W |
46.67 |
42.70 |
-3.97 |
| 6 |
Robinson, Sara C (11)
|
NISDSC |
46.13 |
42.97 |
-3.16 |
| 7 |
Bugbee, Kyla C (12)
|
NIPMSC |
49.16 |
43.90 |
-5.26 |
| 8 |
Share, Elizabeth A (11)
|
NIPMSC |
46.65 |
45.12 |
-1.53 |
| 9 |
Kelley, Michaela G (11)
|
NIGRSC |
47.81 |
46.59 |
-1.22 |
| 10 |
Stonehouse, Sierra L (12)
|
NIGRSC |
49.57 |
47.15 |
-2.42 |
| 11 |
Delehanty, Maeve K (11)
|
NIRRST |
|
47.18 |
- |
| 12 |
Magnera, Sofia A (11)
|
NIWEBS |
46.56 |
47.63 |
1.07 |
| 13 |
Kuhlkin, Elizabeth C (11)
|
NIGCOM |
53.65 |
48.20 |
-5.45 |
| 14 |
Osborne, Samantha R (12)
|
NIGRSC |
55.09 |
49.36 |
-5.73 |
| 14 |
Buzzell, Ella L (12)
|
NIGRSC |
56.76 |
49.36 |
-7.40 |
| 16 |
Beutner, Ella R (11)
|
NIQUIK |
|
51.40 |
- |
| 17 |
Bartholomay, Meghan E (12)
|
NIGCOM |
49.77 |
52.63 |
2.86 |
| 18 |
Kenny, Elisabeth A (11)
|
NIQUIK |
55.31 |
54.44 |
-0.87 |
| 19 |
Cady, Makenna N (11)
|
NIWEBS |
1:07.00 |
55.35 |
-11.65 |
| 20 |
Thoen, Sarah G (11)
|
NISDSC |
1:02.42 |
1:04.61 |
2.19 |
|
Raimondo, Erin E (11)
|
NIGRSC |
|
DQ |
- |
|
Dibble, Peyton A (11)
|
NIWEBS |
57.53 |
NS |
- |
|
Janssen, Kayla L (11)
|
NIWEBS |
57.54 |
NS |
- |