| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crye, Joleigh C (14)
|
NIMERC |
54.10 |
58.72 |
4.62 |
| 2 |
Bryce, Julia M (14)
|
NISDSC |
59.94 |
59.72 |
-0.22 |
| 3 |
Pathammavong, Suriyathai A (14)
|
NIVICT |
58.33 |
1:00.69 |
2.36 |
| 4 |
Bryce, Emma L (16)
|
NISDSC |
1:00.14 |
1:04.01 |
3.87 |
| 5 |
Daley, Regan D (15)
|
NIIRON |
1:02.37 |
1:04.05 |
1.68 |
| 6 |
Austin, Katie L (16)
|
NIMERC |
|
1:04.21 |
- |
| 7 |
Harris, Saiya'Kaede Z (14)
|
NIRRST |
1:08.62 |
1:06.64 |
-1.98 |
| 8 |
Oh, Emily M (16)
|
NISDSC |
1:37.27 |
1:06.97 |
-30.30 |
| 9 |
Robertson, Julia E (14)
|
NIRRST |
1:09.39 |
1:08.71 |
-0.68 |
| 10 |
Nadarajan, Gouripriya (13)
|
NIVELO |
1:13.73 |
1:10.13 |
-3.60 |
| 11 |
Werner, Riley K (13)
|
NIVICT |
1:10.76 |
1:10.56 |
-0.20 |
| 12 |
Davison, Asella M (15)
|
NISDSC |
1:11.24 |
1:12.90 |
1.66 |
| 13 |
Schicker, Emily M (13)
|
NIGRSC |
|
1:37.48 |
- |