| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holmes, Leah J (6)
|
MATWAC |
55.13 |
49.85 |
-5.28 |
| 2 |
Burris, Cecilia R (6)
|
MAFCA |
58.97 |
50.24 |
-8.73 |
| 3 |
Heebner-Elliott, Piper P (6)
|
MAGRA |
53.77 |
52.74 |
-1.03 |
| 4 |
Sawka, Violet A (6)
|
MAUDAC |
59.87 |
52.97 |
-6.90 |
| 5 |
Laliotis, Catherine L (6)
|
MABLUE |
56.97 |
53.91 |
-3.06 |
| 6 |
Yerk, Alexandra L (6)
|
MALVA |
57.39 |
55.63 |
-1.76 |
| 7 |
Melchior, Ella R (5)
|
MAGPAC |
1:01.54 |
58.73 |
-2.81 |
| 8 |
McCall, Anastazia E (6)
|
MADST |
1:00.46 |
59.92 |
-0.54 |
| 9 |
Smyth, Cora E (6)
|
MADST |
58.42 |
1:00.36 |
1.94 |
| 10 |
Ellis, Jordyn K (6)
|
MABLUE |
1:08.55 |
1:04.92 |
-3.63 |
| 11 |
Hauptman, Becca J (6)
|
MAFSA |
1:18.12 |
1:09.76 |
-8.36 |
| 12 |
Carter, Calli L (5)
|
MADST |
1:23.45 |
1:10.06 |
-13.39 |
|
Fink, Liliana G (6)
|
MALVA |
1:04.07 |
DQ |
- |
|
Nordberg, Alaina R (6)
|
MAWWC |
58.83 |
NS |
- |