| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Park, Anna (11)
|
NJSCAR |
40.70 |
40.22 |
-0.48 |
| 2 |
Akram, Aleena A (12)
|
NJSCAR |
41.40 |
41.81 |
0.41 |
| 3 |
Liang, Annie (12)
|
NJSCAR |
44.67 |
41.82 |
-2.85 |
| 4 |
Principe, Julia (12)
|
NJSKYY |
41.05 |
41.90 |
0.85 |
| 5 |
Peel, Juliette D (11)
|
NJXCEL |
44.35 |
42.08 |
-2.27 |
| 6 |
Kim, Noelle (12)
|
NJBB |
42.58 |
42.75 |
0.17 |
| 7 |
Bendoraitis, Elizabeth C (12)
|
NJUNSC |
43.05 |
43.84 |
0.79 |
| 8 |
Frukhtman, Fiana (12)
|
NJSCAR |
47.62 |
44.20 |
-3.42 |
| 9 |
Michaels, Kendall L (11)
|
NJSTAC |
44.58 |
45.45 |
0.87 |
| 10 |
Viola, Gianna (11)
|
NJSTAC |
44.26 |
46.03 |
1.77 |
| 11 |
Atienza, Ava K (11)
|
NJSCAR |
49.31 |
46.19 |
-3.12 |
| 11 |
Macalintal, Madison G (11)
|
NJSCAR |
46.64 |
46.19 |
-0.45 |
| 13 |
Cavlov, Megan E (12)
|
NJBB |
47.09 |
46.87 |
-0.22 |
| 14 |
Kim, Amaris (12)
|
NJSCAR |
47.07 |
47.99 |
0.92 |
| 15 |
Skinner, Cassandra (11)
|
NJSKYY |
50.26 |
48.18 |
-2.08 |
| 16 |
Geller, Macy L (12)
|
NJXCEL |
48.23 |
49.08 |
0.85 |
| 17 |
Eodice, Allison M (11)
|
NJSCAR |
50.65 |
49.15 |
-1.50 |
| 18 |
Chadha, Rhea (12)
|
NJFOBY |
46.91 |
50.16 |
3.25 |
| 19 |
Chari, Maya A (12)
|
NJBB |
50.83 |
51.46 |
0.63 |
| 20 |
Ferat, Ella V (11)
|
NJSCAR |
59.67 |
1:01.21 |
1.54 |