| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shipe, Bryony (11)
|
AMUN-R |
2:58.00 |
2:56.11 |
-1.89 |
| 2 |
Massella, Mollie J (12)
|
AMSRST |
3:10.23 |
2:56.30 |
-13.93 |
| 3 |
Plank, Cora J (12)
|
AMSRST |
3:08.87 |
2:56.32 |
-12.55 |
| 4 |
Shipe, Dylayn (11)
|
AMUN-R |
3:05.73 |
3:00.84 |
-4.89 |
| 5 |
Oates, Amelia M (12)
|
AMFAST |
3:00.05 |
3:01.51 |
1.46 |
| 6 |
Haas, Abigail D (11)
|
AMKW |
3:00.33 |
3:02.68 |
2.35 |
| 7 |
Gauselmann, Madison M (11)
|
AMKW |
3:07.67 |
3:05.26 |
-2.41 |
| 8 |
Alexander, Gabrielle C (12)
|
AMSVSC |
3:07.59 |
3:05.89 |
-1.70 |
| 9 |
Wienand, Paige L (12)
|
AMMMSA |
3:17.31 |
3:16.27 |
-1.04 |
| 10 |
Szymanski, Tessa A (11)
|
AMBYAC |
3:10.41 |
3:17.56 |
7.15 |
| 11 |
Chapman, Grace C (11)
|
AMMLAC |
3:26.03 |
3:20.08 |
-5.95 |
| 12 |
Fogle, Evangeline G (12)
|
AMBCAT |
2:58.83 |
3:22.85 |
24.02 |
| 13 |
Miller, Laurana E (11)
|
AMMMSA |
3:26.21 |
3:23.63 |
-2.58 |