| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schomaker, Charlotte J (7)
|
AMNHA |
27.44 |
25.17 |
-2.27 |
| 2 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
27.31 |
25.34 |
-1.97 |
| 3 |
Capp, Molly L (7)
|
AMMOON |
30.03 |
29.59 |
-0.44 |
| 4 |
Turner, Alyssa J (7)
|
AMRACE |
32.27 |
30.97 |
-1.30 |
| 5 |
Cegan, Olivia R (7)
|
AMHD |
38.98 |
34.00 |
-4.98 |
| 6 |
Coffman, Margaret J (7)
|
AMANSC |
38.30 |
34.27 |
-4.03 |
|
Berke, Mikayla R (7)
|
AMHD |
45.98 |
DQ |
- |
|
Page, Brynley J (7)
|
AMHD |
38.98 |
DQ |
- |
|
Radomski, Annabelle M (7)
|
AMHD |
33.98 |
DQ |
- |
|
Brunner, Tessa G (7)
|
AMPEAQ |
29.00 |
DQ |
- |
|
Chandiran, Sai Anshika (7)
|
AMPEAQ |
35.12 |
DQ |
- |
|
Ressler, Shyloh W (7)
|
AMPRA |
32.47 |
NS |
- |