| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaguzis, Peri S (14)
|
AMKW |
5:14.61 |
5:26.48 |
11.87 |
| 2 |
Shao, Sophie W (14)
|
AMKW |
5:29.98 |
5:33.01 |
3.03 |
| 3 |
Van Deusen, Rosalie A (14)
|
AMGPAC |
5:51.33 |
5:34.83 |
-16.50 |
| 4 |
Little, Anna R (14)
|
AMNAC |
5:48.45 |
5:43.22 |
-5.23 |
| 5 |
Smith, Claudia J (13)
|
AMKW |
6:28.56 |
6:12.28 |
-16.28 |
| 6 |
Georgagis, Anastasia E (14)
|
AMBVSC |
5:56.75 |
6:23.48 |
26.73 |
| 7 |
Dering, Anna R (13)
|
AMKW |
6:24.70 |
6:27.32 |
2.62 |
| 8 |
Meyers, Mackenzie E (13)
|
AMEFSC |
6:30.84 |
6:28.81 |
-2.03 |
| 9 |
Smith, Ally C (14)
|
AMSVSC |
6:30.54 |
6:36.22 |
5.68 |
| 10 |
Gifford, Adelie T (14)
|
AMSVSC |
6:31.55 |
6:38.61 |
7.06 |
| 11 |
Bixler, Willow T (14)
|
AMFAST |
7:22.98 |
6:40.47 |
-42.51 |
| 12 |
Pratt, Riley M (14)
|
AMKW |
6:30.02 |
6:40.68 |
10.66 |
| 13 |
Anderson, Braleigh A (14)
|
AMBPR |
6:17.19 |
6:53.94 |
36.75 |
| 14 |
Berko, Hannah E (14)
|
AMMMSA |
8:01.99 |
7:10.96 |
-51.03 |