| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rusche, Margaret M (13)
|
AMKW |
2:50.02 |
2:46.87 |
-3.15 |
| 2 |
Nardozzi, Elise M (13)
|
AMNGAC |
2:54.19 |
2:52.61 |
-1.58 |
| 3 |
Gatesman, Molly R (14)
|
AMGPAC |
2:54.82 |
2:53.64 |
-1.18 |
| 4 |
Dering, Anna R (13)
|
AMKW |
3:00.02 |
2:53.82 |
-6.20 |
| 5 |
LaRoche, Francesca L (13)
|
AMWHAT |
2:49.42 |
2:54.75 |
5.33 |
| 6 |
Geary, Sydney M (13)
|
AMSFAC |
3:00.50 |
2:55.91 |
-4.59 |
| 7 |
Wen, Gloria Y (13)
|
AMKW |
3:03.11 |
2:56.71 |
-6.40 |
| 8 |
Walko, Julia F (13)
|
AMNAC |
3:07.17 |
3:01.47 |
-5.70 |
| 9 |
Pratt, Riley M (14)
|
AMKW |
2:59.89 |
3:02.91 |
3.02 |
| 10 |
DiMaio, Eva (13)
|
AMUN-R |
2:37.80 |
3:02.94 |
25.14 |
| 11 |
Plank, Anna R (13)
|
AMSRST |
3:08.23 |
3:03.49 |
-4.74 |
| 12 |
Strotmeyer, Kalina S (13)
|
AMMLAC |
3:13.08 |
3:04.80 |
-8.28 |
| 13 |
McKelvey, Kendall O (13)
|
AMNGAC |
3:07.43 |
3:05.26 |
-2.17 |
| 14 |
Murphy, Sierra E (14)
|
AMMMSA |
3:16.89 |
3:06.37 |
-10.52 |
| 14 |
Dunn, Riley N (14)
|
AMTRIN |
3:12.37 |
3:06.37 |
-6.00 |
| 16 |
Haas, Katherine R (13)
|
AMKW |
3:09.90 |
3:07.41 |
-2.49 |
| 17 |
Bixler, Willow T (14)
|
AMFAST |
3:14.62 |
3:15.38 |
0.76 |
| 18 |
Antonova, Maria (13)
|
AMMLAC |
2:48.74 |
3:17.96 |
29.22 |
| 19 |
Osullivan, Avery D (13)
|
AMTRIN |
3:15.33 |
3:25.77 |
10.44 |
|
Pasquella, Ariana D (14)
|
AMKW |
3:07.13 |
DQ |
- |