| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rusche, Margaret M (12)
|
AMKW |
3:08.20 |
2:55.32 |
-12.88 |
| 2 |
Rothrock, Lena R (12)
|
AMSVSC |
2:49.88 |
2:59.09 |
9.21 |
| 3 |
Haas, Abigail D (11)
|
AMKW |
3:25.02 |
3:02.79 |
-22.23 |
| 4 |
Dietz, Sonya E (12)
|
AMSVSC |
2:56.13 |
3:02.98 |
6.85 |
| 5 |
Oates, Amelia M (11)
|
AMFAST |
3:11.99 |
3:04.78 |
-7.21 |
| 6 |
Price, Brooke A (11)
|
AMSPY |
3:13.84 |
3:09.37 |
-4.47 |
| 7 |
Alexander, Gabrielle C (12)
|
AMSVSC |
3:10.03 |
3:12.50 |
2.47 |
| 8 |
Gauselmann, Madison M (11)
|
AMKW |
3:20.02 |
3:19.90 |
-0.12 |
|
Tweed, Abigail G (12)
|
AMBCAT |
2:40.22 |
DQ |
- |
|
Marculescu, Cristina (12)
|
AMKW |
3:15.02 |
DQ |
- |
|
Hensberger, Emma A (12)
|
AMSVSC |
4:01.03 |
DQ |
- |
|
Plank, Cora J (12)
|
AMSRST |
3:08.15 |
DQ |
- |
|
Allen, Julina M (11)
|
AMWAAC |
3:29.97 |
DQ |
- |