| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hoy, Abagail R (7)
|
AMFAST |
29.66 |
18.44 |
-11.22 |
| 2 |
Hull, Naomi D (7)
|
AMFAST |
19.55 |
19.48 |
-0.07 |
| 3 |
Kovacs, Myla M (7)
|
AMMMSA |
21.42 |
21.48 |
0.06 |
| 4 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
22.38 |
21.79 |
-0.59 |
| 5 |
Rideau, Ana M (7)
|
AMMMSA |
24.52 |
22.14 |
-2.38 |
| 6 |
Hunsinger, Sadie G (7)
|
AMFAST |
24.14 |
23.38 |
-0.76 |
| 7 |
Proie, Alexandra S (7)
|
AMPRA |
28.53 |
25.31 |
-3.22 |
| 8 |
Peace, Gia A (7)
|
AMCSC |
27.27 |
25.83 |
-1.44 |
| 9 |
Costanzo, Channing J (7)
|
AMCSC |
31.32 |
26.75 |
-4.57 |
| 10 |
Bittner, Mia A (7)
|
AMRXA |
39.99 |
26.97 |
-13.02 |
| 11 |
Wang, Angela L (7)
|
AMFAST |
29.99 |
28.04 |
-1.95 |
| 12 |
Pipes, Kayley G (7)
|
AMWAAC |
46.45 |
30.25 |
-16.20 |
| 13 |
Ward, Ariana R (7)
|
AMWAAC |
32.66 |
30.86 |
-1.80 |
| 14 |
Sudol, Layla A (7)
|
AMMMSA |
33.99 |
32.82 |
-1.17 |
|
Coffman, Margaret J (7)
|
AMANSC |
30.32 |
NS |
- |
|
Eichelberger, Maragret J (7)
|
AMFAST |
30.99 |
NS |
- |