| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Manjerovic, Anna M (13)
|
NIPACK |
41.55 |
38.20 |
-3.35 |
| 2 |
Bedford, Ainsley N (12)
|
NIWEBS |
39.24 |
39.30 |
0.06 |
| 3 |
Flanigen, Ruby J (11)
|
NIIRON |
42.66 |
41.34 |
-1.32 |
| 4 |
Robinson, Sara C (11)
|
NISDSC |
42.97 |
42.09 |
-0.88 |
| 5 |
Guagliardo, Giovanna (12)
|
NIIRON |
44.26 |
42.77 |
-1.49 |
| 6 |
Johnson, Ila A (12)
|
NIIRON |
44.79 |
43.18 |
-1.61 |
| 7 |
Bedford, Marin B (10)
|
NIWEBS |
43.02 |
44.40 |
1.38 |
| 8 |
Williams, Tory L (13)
|
NIPACK |
43.27 |
46.46 |
3.19 |
| 9 |
Maas, Kaylee A (9)
|
NIIRON |
48.70 |
47.61 |
-1.09 |
| 10 |
Huerta-Andrade, Isabella (11)
|
NIPACK |
53.34 |
48.57 |
-4.77 |
| 11 |
Selioutski, Leeva A (13)
|
NISDSC |
51.00 |
48.69 |
-2.31 |
| 12 |
Gallant, Isabella A (9)
|
NIPACK |
50.90 |
50.45 |
-0.45 |
| 13 |
Zhang, Maureen X (10)
|
NIPACK |
56.03 |
52.27 |
-3.76 |
| 14 |
Magnera, Alaura (9)
|
NIWEBS |
1:00.04 |
56.30 |
-3.74 |
| 15 |
Wagner, Keaghan J (10)
|
NISDSC |
1:00.37 |
59.41 |
-0.96 |
| 16 |
Cunniffe, Chloe L (9)
|
NIPACK |
|
1:00.78 |
- |
| 17 |
Wagner, Ryleigh J (7)
|
NISDSC |
1:41.23 |
1:01.93 |
-39.30 |
| 18 |
Gonzalez, Alexis W (9)
|
NIPACK |
1:06.97 |
1:02.51 |
-4.46 |
| 19 |
Van Hout, Kennyn A (8)
|
NISDSC |
1:04.84 |
1:03.17 |
-1.67 |
| 20 |
Considine, Elizabeth A (8)
|
NIWEBS |
1:29.00 |
1:28.70 |
-0.30 |
|
Garigen, Natalie K (8)
|
NIWEBS |
1:14.03 |
DQ |
- |
|
Terio, Chloe M (8)
|
NIPACK |
|
NS |
- |
|
Garnot, Madelyne R (14)
|
NIIRON |
46.90 |
NS |
- |