| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Shea, Connor J (16)
|
NIFAST |
29.69 |
29.03 |
-0.66 |
| 2 |
Zani, Samuel A (16)
|
NIIRON |
33.19 |
32.87 |
-0.32 |
| 3 |
Brett, Liam J (12)
|
NIPACK |
37.76 |
39.26 |
1.50 |
| 4 |
Peters, Ryan R (14)
|
NIGCOM |
42.45 |
42.14 |
-0.31 |
| 5 |
Zappia, Luke H (11)
|
NIPACK |
48.93 |
46.20 |
-2.73 |
| 6 |
Jiang, Shidao (12)
|
NIGCOM |
|
46.93 |
- |
| 7 |
Straub, William C (11)
|
NIPACK |
45.65 |
47.02 |
1.37 |
| 8 |
Merkl, Aaron W (13)
|
NIGCOM |
58.08 |
49.60 |
-8.48 |
| 9 |
Rossetti, Maxwell R (12)
|
NIPACK |
|
49.76 |
- |
| 10 |
Mattingly, Ian T (10)
|
NIPACK |
59.14 |
51.03 |
-8.11 |
| 11 |
Coccia, Bryson A (9)
|
NIGCOM |
54.38 |
51.72 |
-2.66 |
| 12 |
Madison, Benjamin M (11)
|
NIWEBS |
48.97 |
52.53 |
3.56 |
| 13 |
Cottone, Cameron A (11)
|
NIGCOM |
52.60 |
55.36 |
2.76 |
| 14 |
Madison, Owen P (10)
|
NIWEBS |
1:20.00 |
57.40 |
-22.60 |
| 15 |
Belous, Matthew I (10)
|
NIPACK |
1:05.60 |
58.05 |
-7.55 |
| 16 |
Mattingly, Austin P (8)
|
NIPACK |
|
1:05.46 |
- |
| 17 |
Kumar, Dwaraka S (9)
|
NIWEBS |
1:10.00 |
1:06.50 |
-3.50 |
|
Blanchard, Zachary E (12)
|
NIFAST |
1:06.57 |
DQ |
- |
|
Straub, Alexander M (8)
|
NIPACK |
|
DQ |
- |
|
Loomis, Noah W (12)
|
NIIRON |
1:05.00 |
DQ |
- |
|
Kalkounis, GeorgeAnthony (16)
|
NIWEBS |
50.00 |
DQ |
- |