| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dinh, Nakisha V (12)
|
NIWEBS |
1:03.33 |
1:03.25 |
-0.08 |
| 2 |
Cooper, Sarah C (15)
|
NIPACK |
1:00.56 |
1:04.51 |
3.95 |
| 3 |
Kirchgessner, Robin K (18)
|
NIIRON |
1:00.15 |
1:04.82 |
4.67 |
| 4 |
Wood, Alicia G (15)
|
NIIRON |
1:02.20 |
1:05.41 |
3.21 |
| 5 |
Xu, Ming Na (12)
|
NIFAST |
1:06.82 |
1:05.69 |
-1.13 |
| 6 |
Nguyen, Vivian A (12)
|
NIWEBS |
1:05.70 |
1:06.83 |
1.13 |
| 7 |
Huang, Katherine (15)
|
NIPACK |
1:04.60 |
1:07.73 |
3.13 |
| 8 |
Shen, Alivia (11)
|
NIPACK |
1:11.98 |
1:10.69 |
-1.29 |
| 9 |
Corby, Meghan J (15)
|
NIPACK |
1:17.52 |
1:12.95 |
-4.57 |
| 10 |
Audi, Lauren A (15)
|
NIWEBS |
1:11.54 |
1:16.49 |
4.95 |
| 11 |
Healy, Catherine M (14)
|
NIWEBS |
1:22.53 |
1:19.84 |
-2.69 |
| 12 |
Gallegos, Lena P (12)
|
NIFAST |
1:21.18 |
1:21.97 |
0.79 |
| 13 |
Willis, Kylie E (10)
|
NIWEBS |
1:45.00 |
1:28.62 |
-16.38 |
| 14 |
Harrington, Payton V (13)
|
NIFAST |
1:33.75 |
1:34.97 |
1.22 |
| 15 |
Hartley, Elliana G (10)
|
NIFAST |
1:54.25 |
1:38.54 |
-15.71 |
| 16 |
Maas, Kaylee A (9)
|
NIIRON |
|
1:45.72 |
- |
| 17 |
Buck, Kathryn E (12)
|
NIWEBS |
2:00.00 |
1:48.50 |
-11.50 |
| 18 |
Orlando, Lillian G (10)
|
NIPACK |
|
1:59.32 |
- |
|
Krenzer, Emma E (16)
|
NIIRON |
1:23.00 |
DQ |
- |
|
Cady, Makenna N (12)
|
NIWEBS |
1:59.58 |
NS |
- |