| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Naccarella, Maria E (15)
|
NIWEBS |
30.15 |
29.67 |
-0.48 |
| 2 |
Nenadic, Maria G (16)
|
NIFAST |
28.60 |
30.21 |
1.61 |
| 3 |
Romanick, Lauren A (15)
|
NIPACK |
31.40 |
31.88 |
0.48 |
| 4 |
Branch, Alison G (16)
|
NIIRON |
38.31 |
33.40 |
-4.91 |
| 5 |
Feck, Hannah R (14)
|
NIPACK |
34.00 |
36.29 |
2.29 |
| 6 |
Huerta-Andrade, Isabella (11)
|
NIPACK |
40.74 |
36.93 |
-3.81 |
| 7 |
Smeenk, Anneke T (13)
|
NISDSC |
41.33 |
37.54 |
-3.79 |
| 8 |
Minnigh, Ella N (12)
|
NIPACK |
45.54 |
43.46 |
-2.08 |
| 9 |
Gallant, Isabella A (9)
|
NIPACK |
46.44 |
44.20 |
-2.24 |
| 10 |
Hicks, Rebecca N (11)
|
NIPACK |
45.09 |
44.27 |
-0.82 |
| 11 |
Patel, Sia M (11)
|
NIPACK |
44.67 |
45.75 |
1.08 |
| 12 |
Zhang, Maureen X (10)
|
NIPACK |
52.15 |
46.59 |
-5.56 |
| 13 |
Wagner, Ryleigh J (7)
|
NISDSC |
51.52 |
48.13 |
-3.39 |
| 14 |
Weber, Sophia R (7)
|
NIWEBS |
1:05.00 |
52.94 |
-12.06 |
| 15 |
Quinto, Lilian M (8)
|
NIIRON |
56.12 |
54.09 |
-2.03 |
| 16 |
Maaraba, Hanna M (8)
|
NIPACK |
|
59.88 |
- |
| 17 |
Gonzalez, Alexis W (9)
|
NIPACK |
56.97 |
1:00.40 |
3.43 |
| 18 |
Considine, Elizabeth A (8)
|
NIWEBS |
1:02.35 |
1:03.19 |
0.84 |
|
Selioutski, Leeva A (13)
|
NISDSC |
49.00 |
DQ |
- |
|
Bedford, Marin B (10)
|
NIWEBS |
42.63 |
DQ |
- |
|
Garigen, Natalie K (8)
|
NIWEBS |
50.52 |
DQ |
- |