| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ruhl, Jonah W (11)
|
NTATAC |
32.38 |
31.47 |
-0.91 |
| 2 |
Tobin, Michael I (12)
|
NTATAC |
36.03 |
36.57 |
0.54 |
| 3 |
Roman-Bocanegra, Carlos R (11)
|
NTCOPS |
39.80 |
37.28 |
-2.52 |
| 4 |
Jadhav, Ranveer K (12)
|
NTCOPS |
45.77 |
39.79 |
-5.98 |
| 5 |
Potter, Daniel V (12)
|
NTCOPS |
40.16 |
39.83 |
-0.33 |
| 6 |
Tan, Cory E (11)
|
NTCOPS |
43.02 |
40.01 |
-3.01 |
| 7 |
Abshire, Andrew (11)
|
NTATAC |
44.47 |
42.51 |
-1.96 |
| 8 |
Trayer, Connor D (11)
|
NTCOPS |
41.87 |
42.57 |
0.70 |
| 9 |
Bishop, Sterling A (11)
|
NTCOPS |
43.78 |
43.50 |
-0.28 |
| 10 |
Gandhi, Xerxes (12)
|
NTCOPS |
45.29 |
45.55 |
0.26 |
| 11 |
Shah, Eshaan R (11)
|
NTCOPS |
41.57 |
46.03 |
4.46 |
| 12 |
Zaballa, Sebastian T (11)
|
NTCOPS |
46.19 |
46.68 |
0.49 |
| 13 |
Wagoner, Nathan A (11)
|
NTCOPS |
|
47.92 |
- |