| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ziegler, Abigail J (11)
|
ILFVFY |
32.20 |
31.77 |
-0.43 |
| 2 |
Maratea, Kylie A (12)
|
ILFVFY |
32.23 |
31.79 |
-0.44 |
| 3 |
Schoenfielder, Isabella G (12)
|
ILFVFY |
35.35 |
35.86 |
0.51 |
| 4 |
Forrester, Nyeema (12)
|
ILNAPY |
37.89 |
36.24 |
-1.65 |
| 5 |
Brewer, Lainey E (12)
|
ILFVFY |
36.16 |
36.40 |
0.24 |
| 6 |
Schultz, Victoria E (12)
|
ILFVFY |
36.55 |
37.53 |
0.98 |
| 7 |
Sanchez, Adrianna M (12)
|
ILFVFY |
40.45 |
38.37 |
-2.08 |
| 8 |
Ruparel, Rakshita (11)
|
ILNAPY |
40.27 |
38.79 |
-1.48 |
| 9 |
Jordan, Bernadette M (11)
|
ILFVFY |
43.17 |
40.98 |
-2.19 |
| 10 |
Murray, Janiah Z (11)
|
ILFVFY |
40.15 |
41.81 |
1.66 |