| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chambers, Tatum R (16)
|
NTSTAR |
25.40 |
26.39 |
0.99 |
| 2 |
Jones, Lauren S (16)
|
NTCOPS |
26.05 |
26.47 |
0.42 |
| 3 |
McCoppin, Claire E (16)
|
NTSTAR |
29.02 |
27.91 |
-1.11 |
| 4 |
Feeney, Holland A (15)
|
NTSTAR |
27.90 |
28.44 |
0.54 |
| 5 |
Whitmer, Katherine P (17)
|
NTCOPS |
27.30 |
28.45 |
1.15 |
| 6 |
McCormick, Darby J (16)
|
NTSTAR |
28.65 |
28.47 |
-0.18 |
| 7 |
Zevallos, Ava G (16)
|
NTSTAR |
29.25 |
29.08 |
-0.17 |
| 8 |
Suwanda, Mariska G (16)
|
NTCOPS |
26.68 |
29.18 |
2.50 |
| 9 |
Mather, Hannah E (16)
|
NTSTAR |
27.69 |
29.57 |
1.88 |
| 10 |
Jaimes, Alexandria C (16)
|
NTSTAR |
31.25 |
30.34 |
-0.91 |
|
Osborn, Taylor G (17)
|
NTCOPS |
24.63 |
NS |
- |