| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Maguire, Grace A (17)
|
NEBGSC |
33.37 |
32.45 |
-0.92 |
| 2 |
Contich, Jillian J (18)
|
FGWASC |
32.63 |
33.25 |
0.62 |
| 3 |
Rhodes, Jillian D (17)
|
NEBGSC |
35.64 |
33.43 |
-2.21 |
| 4 |
Dressel, Sydnee P (17)
|
WIEBSC |
33.47 |
33.67 |
0.20 |
| 5 |
Gearan, Jessica M (17)
|
NEBGSC |
32.68 |
33.81 |
1.13 |
| 6 |
Moss, Faith E (21)
|
FGWASC |
32.17 |
33.98 |
1.81 |
| 7 |
McCormack, Ciara M (17)
|
NEBGSC |
35.87 |
35.29 |
-0.58 |
| 8 |
Flagiello, Olivia (17)
|
MRTRI |
35.48 |
35.36 |
-0.12 |
| 9 |
Downey, Skye E (21)
|
FGPST |
33.90 |
35.82 |
1.92 |
| 10 |
Mitchell, Kelly M (17)
|
NEBGSC |
36.66 |
35.88 |
-0.78 |
| 11 |
Legge, Vanessa (19)
|
ZZMPM |
34.98 |
36.17 |
1.19 |
| 12 |
Pacitti, Alexandra L (20)
|
FGPST |
34.49 |
36.20 |
1.71 |
| 13 |
Stockford, Karlie m (17)
|
NEWTST |
35.91 |
37.62 |
1.71 |
| 14 |
McCarthy, Delaney M (17)
|
ILMAC |
|
37.93 |
- |
| 15 |
Horvath, Anna R (17)
|
WIEBSC |
37.88 |
38.42 |
0.54 |
| 16 |
Cunningham, Hannah M (17)
|
ILMAC |
|
41.22 |
- |
| 17 |
Hernandez, Raeana M (17)
|
FGCCPR |
43.58 |
43.40 |
-0.18 |
|
Abad, Victoria A (17)
|
FGPST |
34.44 |
NS |
- |
|
Manrique, Camila (19)
|
FGPST |
34.55 |
NS |
- |