| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schaetz, Emma J (17)
|
WIEBSC |
30.06 |
30.49 |
0.43 |
| 2 |
Walsh, Katelyn K (17)
|
MRPATS |
30.80 |
30.52 |
-0.28 |
| 3 |
Reyes, Helina (17)
|
ZZLACE |
31.48 |
30.98 |
-0.50 |
| 4 |
McCormack, Ciara M (17)
|
NEBGSC |
33.07 |
31.48 |
-1.59 |
| 5 |
Pacitti, Alexandra L (20)
|
FGPST |
30.63 |
32.38 |
1.75 |
| 6 |
Flagiello, Mia (17)
|
MRTRI |
35.90 |
32.49 |
-3.41 |
| 7 |
Oliva, Sophia M (21)
|
FGPST |
30.10 |
32.61 |
2.51 |
| 8 |
Moss, Faith E (21)
|
FGWASC |
30.42 |
32.63 |
2.21 |
| 9 |
Mitchell, Kelly M (17)
|
NEBGSC |
33.01 |
32.66 |
-0.35 |
| 10 |
Stockford, Karlie m (17)
|
NEWTST |
31.59 |
33.13 |
1.54 |
| 11 |
Hrdlick, Gabrielle L (17)
|
WIEBSC |
33.80 |
33.29 |
-0.51 |
| 12 |
Contich, Jillian J (18)
|
FGWASC |
31.33 |
33.95 |
2.62 |
| 13 |
Cunningham, Hannah M (17)
|
ILMAC |
|
34.59 |
- |
| 14 |
McCarthy, Delaney M (17)
|
ILMAC |
|
34.94 |
- |
| 15 |
Crooks, Gabriella L (17)
|
VASEVA |
35.64 |
35.29 |
-0.35 |
| 16 |
Boehm, Janelle A (17)
|
WIEBSC |
47.93 |
35.35 |
-12.58 |
| 17 |
Hernandez, Raeana M (17)
|
FGCCPR |
39.38 |
40.76 |
1.38 |