| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pupke, Cameron R (11)
|
MRWISC |
35.71 |
33.76 |
-1.95 |
| 2 |
Brink, Keelyn R (11)
|
MRWISC |
34.54 |
34.00 |
-0.54 |
| 3 |
Benavides, Genesis S (12)
|
MRWISC |
35.69 |
35.25 |
-0.44 |
| 4 |
Kunca, Mikalah A (11)
|
MRCFJV |
35.69 |
35.99 |
0.30 |
| 5 |
Neary, Madison G (12)
|
MRWISC |
37.84 |
37.59 |
-0.25 |
| 6 |
Yates, Jordyn A (12)
|
MRMHHW |
39.40 |
37.91 |
-1.49 |
| 6 |
Hayes, Cassidy C (11)
|
MRWISC |
39.71 |
37.91 |
-1.80 |
| 8 |
Madison, Lucinda M (11)
|
MRWISC |
40.93 |
38.26 |
-2.67 |
| 9 |
Harrison, Violet T (12)
|
MRWISC |
43.05 |
42.14 |
-0.91 |
| 10 |
Amato, Esther (11)
|
MRCFJV |
44.06 |
44.63 |
0.57 |
| 11 |
Ali, Katina L (11)
|
MRWISC |
45.99 |
45.11 |
-0.88 |
| 12 |
Klempner, Morgan (12)
|
MRCFJV |
50.01 |
49.42 |
-0.59 |
| 13 |
Cabrera-Hill, Angelese A (12)
|
MRRFAC |
56.80 |
54.47 |
-2.33 |