| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pece, Delaney R (11)
|
ADMDST |
34.07 |
34.52 |
0.45 |
| 2 |
Dabo, Mariama F (11)
|
MRHVD |
34.12 |
36.83 |
2.71 |
| 3 |
Vos, Brooke C (11)
|
ADMDST |
37.53 |
37.10 |
-0.43 |
| 4 |
Jarvis, Emma M (12)
|
MRHVD |
37.43 |
37.35 |
-0.08 |
| 5 |
Horgan, Rosemary E (11)
|
MRHVD |
39.55 |
38.79 |
-0.76 |
| 6 |
Nemeth, Ava E (11)
|
MRHVD |
39.22 |
39.72 |
0.50 |
| 7 |
Bautista, Jasmine (12)
|
MRUN |
42.57 |
41.09 |
-1.48 |
| 8 |
Baks, Janneke P (11)
|
MRHVD |
42.23 |
43.33 |
1.10 |
| 9 |
Cabrera Zavala, Alexa M (11)
|
MRUN |
47.89 |
43.35 |
-4.54 |
| 10 |
Perez Quiroz, Brianna Y (12)
|
MRUN |
47.72 |
49.80 |
2.08 |
|
Zeman, Sophia E (11)
|
MRHVD |
54.76 |
DQ |
- |
|
Harrell, Aya (11)
|
MRUN |
51.22 |
DQ |
- |
|
Huang, Alyssa (11)
|
MRCFJV |
39.13 |
NS |
- |