| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pupke, Cameron R (11)
|
MRWISC |
30.99 |
31.67 |
0.68 |
| 2 |
Brink, Keelyn R (11)
|
MRWISC |
31.22 |
31.77 |
0.55 |
| 3 |
Benavides, Genesis S (12)
|
MRWISC |
33.44 |
32.33 |
-1.11 |
| 4 |
Hayes, Cassidy C (11)
|
MRWISC |
38.34 |
36.51 |
-1.83 |
| 5 |
Neary, Madison G (12)
|
MRWISC |
38.43 |
38.01 |
-0.42 |
| 6 |
Yates, Jordyn A (12)
|
MRMHHW |
43.95 |
39.89 |
-4.06 |
| 7 |
Harrison, Violet T (12)
|
MRWISC |
43.32 |
43.76 |
0.44 |
| 8 |
Klempner, Morgan (12)
|
MRCFJV |
52.59 |
46.24 |
-6.35 |
| 9 |
Ali, Katina L (11)
|
MRWISC |
48.99 |
49.95 |
0.96 |
| 10 |
Cabrera-Hill, Angelese A (12)
|
MRRFAC |
52.63 |
52.61 |
-0.02 |