| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fernandez, Eliseo D (12)
|
MRMHHW |
28.15 |
25.67 |
-2.48 |
| 2 |
Tuttle, Peyton J (12)
|
MRNBS |
29.78 |
27.72 |
-2.06 |
| 3 |
Chen-Suzuki, Keizo N (12)
|
MRNDAC |
28.85 |
28.23 |
-0.62 |
| 4 |
Speare, Troy D (11)
|
MRNDAC |
28.99 |
28.82 |
-0.17 |
| 5 |
Button, Samuel M (12)
|
MRNDAC |
29.27 |
29.03 |
-0.24 |
| 6 |
Delatorre, Jael (12)
|
MRMHHW |
29.14 |
29.56 |
0.42 |
| 7 |
De Borba Silva, Lucas C (12)
|
MRRA |
33.88 |
30.18 |
-3.70 |
| 8 |
Roche, James (12)
|
MRRA |
30.72 |
31.11 |
0.39 |
| 8 |
Cooper, Jackson C (12)
|
MRNBS |
31.82 |
31.11 |
-0.71 |
| 10 |
Karadeniz, Derin M (11)
|
MRRFAC |
31.12 |
31.55 |
0.43 |
| 11 |
McCree-Salazar, Caden C (11)
|
MRHVD |
34.44 |
31.64 |
-2.80 |
| 12 |
Pagano, Jax A (12)
|
MRNBS |
33.18 |
31.67 |
-1.51 |
| 13 |
Aldridge, Brian C (12)
|
MRLBA |
33.72 |
32.53 |
-1.19 |
| 14 |
Howard, Andrew J (11)
|
MRHVD |
37.13 |
33.13 |
-4.00 |
| 15 |
Racz, Gregory L (12)
|
MRRA |
33.94 |
33.44 |
-0.50 |
| 16 |
Dorner, Benjamin R (12)
|
MRNDAC |
32.42 |
33.93 |
1.51 |
| 17 |
Constancio, Markus A (12)
|
MRNBS |
37.87 |
35.18 |
-2.69 |
| 18 |
Payne, William M (12)
|
MRRA |
35.97 |
35.49 |
-0.48 |
| 19 |
Chamberlain, Nicholas J (11)
|
MRRFAC |
35.65 |
35.59 |
-0.06 |
| 20 |
Quezada, Louis A (12)
|
MRRA |
37.97 |
38.27 |
0.30 |
| 21 |
Poellot, Reed C (11)
|
MRHVD |
39.70 |
38.58 |
-1.12 |
| 22 |
Korzniewski, Oliver (11)
|
MRLBA |
39.98 |
38.63 |
-1.35 |
| 23 |
Otis, Joshua A (11)
|
MRNDAC |
39.43 |
40.81 |
1.38 |
| 23 |
Calidonio, Mason A (12)
|
MRLBA |
|
40.81 |
- |
| 25 |
Gonzalez, Demetri N (11)
|
MRRA |
1:00.00 |
44.64 |
-15.36 |
| 26 |
Bolz, Liam M (11)
|
MRLBA |
52.25 |
44.77 |
-7.48 |
| 27 |
Hernandez, Oscar (12)
|
MRMHHW |
49.18 |
51.73 |
2.55 |