| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhou, Ethan (11)
|
MRUN |
31.12 |
30.53 |
-0.59 |
| 2 |
Daniels, Owen D (11)
|
MRNDAC |
32.12 |
32.85 |
0.73 |
| 3 |
Delatorre, Jael (12)
|
MRMHH |
39.17 |
36.56 |
-2.61 |
| 4 |
Ruiz, Nathaniel R (11)
|
MRHVD |
35.23 |
36.62 |
1.39 |
| 5 |
Kablaoui, Abdulraouf (12)
|
MRRFAC |
40.56 |
39.59 |
-0.97 |
| 6 |
Heidebrecht, Martin H (12)
|
MRNDAC |
40.92 |
40.63 |
-0.29 |
| 7 |
Dorner, Benjamin (12)
|
MRNDAC |
39.05 |
40.68 |
1.63 |
| 8 |
Chamberlain, Nicholas J (11)
|
MRRFAC |
47.24 |
43.64 |
-3.60 |
| 9 |
Pociask, Joseph V (11)
|
MRNDAC |
44.77 |
44.85 |
0.08 |
| 10 |
Kyaw, Thit-sar (11)
|
MRHVD |
45.94 |
46.04 |
0.10 |
| 11 |
Ramirez, Bryan E (11)
|
MRMHH |
48.30 |
46.75 |
-1.55 |
| 12 |
Cruz Santos, Yoel (12)
|
MRMHH |
51.97 |
51.14 |
-0.83 |