| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Armistead, Allison H (14)
|
MRAG |
26.16 |
27.18 |
1.02 |
| 2 |
Loughran, Megan R (14)
|
MRRFAC |
27.19 |
28.48 |
1.29 |
| 3 |
Murray, Gabriella R (13)
|
MRRFAC |
30.31 |
30.26 |
-0.05 |
| 4 |
Chant, Lucy J (13)
|
MRHVD |
30.67 |
30.88 |
0.21 |
| 5 |
Alcantara, Antonella L (13)
|
MRRFAC |
31.48 |
31.17 |
-0.31 |
| 6 |
Quezada, Julia C (13)
|
MRRFAC |
29.30 |
31.21 |
1.91 |
| 7 |
Lau, Samantha C (13)
|
MRHVD |
31.93 |
31.45 |
-0.48 |
| 8 |
Cordeiro, Alex I (14)
|
MRHVD |
30.53 |
31.70 |
1.17 |
| 9 |
Hogan, Bridget R (13)
|
MRHVD |
33.70 |
33.76 |
0.06 |
| 10 |
Harrington, Gwenyth A (13)
|
MRHVD |
40.40 |
39.47 |
-0.93 |