| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Olsson, Kian J (10)
|
MRNDAC |
46.72 |
43.49 |
-3.23 |
| 2 |
Stueber, Colin J (9)
|
MRWISC |
46.75 |
44.96 |
-1.79 |
| 3 |
Neary, Jack T (9)
|
MRWISC |
56.24 |
50.44 |
-5.80 |
| 4 |
Dorner, Declan M (9)
|
MRNDAC |
52.20 |
50.61 |
-1.59 |
| 5 |
Berardis, Christopher J (9)
|
MRHVD |
57.08 |
50.82 |
-6.26 |
| 6 |
Kui, Barnabas Z (10)
|
MRRFAC |
53.50 |
53.21 |
-0.29 |
| 7 |
Kui, Adam W (10)
|
MRRFAC |
57.43 |
55.12 |
-2.31 |
| 8 |
Stern, Noah I (9)
|
MRLIE |
58.46 |
55.46 |
-3.00 |
| 9 |
Henrichs, Kai L (10)
|
MRLIE |
56.21 |
58.93 |
2.72 |
| 10 |
Markos, Thomas M (10)
|
MRHVD |
|
1:00.30 |
- |
| 11 |
McCree-Salazar, Caden C (10)
|
MRHVD |
|
1:00.51 |
- |
| 12 |
Hewitt, Peter M (9)
|
MRNDAC |
1:05.11 |
1:04.51 |
-0.60 |
| 13 |
Hewitt, Jack D (9)
|
MRNDAC |
1:03.11 |
1:15.00 |
11.89 |
|
Pena, Brandon J (10)
|
MRHVD |
|
DQ |
- |
|
McCaffrey, Mitchell (10)
|
MRLIE |
57.44 |
DQ |
- |
|
Benavides, Isaiah J (9)
|
MRWISC |
57.99 |
DQ |
- |