| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Neary, Jack T (9)
|
MRWISC |
44.53 |
41.99 |
-2.54 |
| 2 |
Stern, Noah I (9)
|
MRLIE |
45.44 |
42.66 |
-2.78 |
| 3 |
Henrichs, Kai L (10)
|
MRLIE |
42.93 |
42.87 |
-0.06 |
| 4 |
Kui, Barnabas Z (10)
|
MRRFAC |
45.32 |
43.00 |
-2.32 |
| 5 |
Howard, Andrew J (10)
|
MRHVD |
42.77 |
45.92 |
3.15 |
| 6 |
Benavides, Isaiah J (9)
|
MRWISC |
52.99 |
46.65 |
-6.34 |
| 7 |
Pena, Brandon J (10)
|
MRHVD |
52.19 |
47.04 |
-5.15 |
| 8 |
Berardis, Christopher J (9)
|
MRHVD |
51.15 |
47.39 |
-3.76 |
| 9 |
McCree-Salazar, Caden C (10)
|
MRHVD |
|
47.86 |
- |
| 10 |
Beaudoin, Jonah D (9)
|
MRHVD |
52.51 |
48.46 |
-4.05 |
| 11 |
Stueber, Colin J (9)
|
MRWISC |
50.25 |
48.74 |
-1.51 |
| 12 |
Chamberlain, Nicholas J (10)
|
MRRFAC |
49.09 |
50.27 |
1.18 |
| 13 |
McCaffrey, Mitchell (10)
|
MRLIE |
51.41 |
51.39 |
-0.02 |
| 14 |
Kui, Adam W (10)
|
MRRFAC |
47.56 |
51.47 |
3.91 |
| 15 |
Hewitt, Peter M (9)
|
MRNDAC |
56.51 |
53.39 |
-3.12 |
| 16 |
Brady, James F (9)
|
MRHVD |
1:07.97 |
1:00.01 |
-7.96 |
| 17 |
Howard, Sam W (9)
|
MRRFAC |
1:00.89 |
1:00.54 |
-0.35 |
| 18 |
Poellot, Reed C (10)
|
MRHVD |
59.12 |
1:01.18 |
2.06 |