| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baker, Olivia A (12)
|
OHLTPY |
30.17 |
29.95 |
-0.22 |
| 2 |
Downey, Madeline P (12)
|
OHLTPY |
33.31 |
32.33 |
-0.98 |
| 3 |
Bowles, Audrey (12)
|
OHDRY |
38.94 |
33.52 |
-5.42 |
| 4 |
Oberoi, Simran C (12)
|
OHLTPY |
34.68 |
33.54 |
-1.14 |
| 5 |
Shawberry, Eiley C (11)
|
OHLTPY |
34.15 |
33.55 |
-0.60 |
| 6 |
Green, Margaret L (12)
|
OHLTPY |
33.71 |
35.25 |
1.54 |
| 7 |
Ginsburg, Lucy R (12)
|
OHDRY |
|
37.69 |
- |
| 8 |
Beck, Katelynn S (12)
|
OHDRY |
|
39.13 |
- |
| 9 |
Ganesh, Samiksha * (11)
|
OHLTPY |
39.64 |
40.32 |
0.68 |
| 10 |
Davis, Hannah G (11)
|
OHLTPY |
33.30 |
40.55 |
7.25 |
| 11 |
Gudla, Sankeertana * (12)
|
OHLTPY |
40.34 |
41.45 |
1.11 |
| 12 |
Sutton, Ainsley E (11)
|
OHLTPY |
44.79 |
44.98 |
0.19 |
| 13 |
Lingampally, Hamsini * (11)
|
OHLTPY |
53.28 |
50.83 |
-2.45 |