| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Franz, Jack M (14)
|
OHLTPY |
54.12 |
54.13 |
0.01 |
| 2 |
Miller, Mason W (14)
|
OHLTPY |
52.18 |
55.49 |
3.31 |
| 3 |
McMasters, William M (13)
|
OHLYST |
54.80 |
56.05 |
1.25 |
| 4 |
Riegler, Eli M (13)
|
OHGYG |
|
57.22 |
- |
| 5 |
Blum, Ryan E (13)
|
RCY |
58.55 |
57.40 |
-1.15 |
| 6 |
Gaier, Michael S (13)
|
OHLTPY |
56.16 |
57.99 |
1.83 |
| 7 |
Blachly, David S (13)
|
OHLTPY |
1:03.91 |
1:01.62 |
-2.29 |
| 8 |
Howard, Lance G (13)
|
OHHURY |
1:04.38 |
1:02.29 |
-2.09 |
| 8 |
Fowler, Brenton (13)
|
OHGCY |
1:07.58 |
1:02.29 |
-5.29 |
| 10 |
Caputo, Anthony (13)
|
OHCHY |
1:07.09 |
1:04.65 |
-2.44 |
| 11 |
Barker, Matthew L (13)
|
OHLTPY |
1:07.01 |
1:05.31 |
-1.70 |
| 12 |
Sebenoler, Thomas D (13)
|
OHLTPY |
1:10.02 |
1:07.28 |
-2.74 |
| 13 |
Rodriguez, Brendon A (13)
|
OHGCY |
1:06.58 |
1:08.97 |
2.39 |
| 14 |
Loughrey, Carson M (13)
|
OHLTPY |
1:09.70 |
1:09.44 |
-0.26 |
| 15 |
Ziliak, Samuel M (13)
|
OHLTPY |
1:07.99 |
1:09.72 |
1.73 |
| 16 |
Kozen, Benjamin K (13)
|
OHMCY |
1:09.52 |
1:10.01 |
0.49 |
| 17 |
Tan, Benjamin W (13)
|
OHLTPY |
1:25.61 |
1:11.73 |
-13.88 |
| 18 |
Kuppa, Anish K (13)
|
OHLTPY |
1:23.83 |
1:19.18 |
-4.65 |
| 19 |
Huang, Daniel H (14)
|
OHLTPY |
1:22.40 |
1:19.34 |
-3.06 |
| 20 |
Thomas, Owen C (13)
|
OHHURY |
1:30.00 |
1:22.87 |
-7.13 |
| 21 |
Pokala, Akshar * (14)
|
OHLTPY |
1:38.05 |
1:32.35 |
-5.70 |