| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Faith J (12)
|
OHDRY |
31.97 |
30.91 |
-1.06 |
| 2 |
Oberoi, Simran C (12)
|
OHLTPY |
33.54 |
32.46 |
-1.08 |
| 3 |
Bowles, Audrey (12)
|
OHDRY |
33.52 |
33.11 |
-0.41 |
| 4 |
Ruholt, Madeline K (11)
|
OHHURY |
34.67 |
33.89 |
-0.78 |
| 5 |
Burdick, Anna J (12)
|
OHHURY |
34.09 |
35.87 |
1.78 |
| 6 |
Bollinger, Brynn M (11)
|
OHHURY |
37.13 |
36.95 |
-0.18 |
| 7 |
Butcher, Chloe H (12)
|
OHHURY |
36.89 |
37.18 |
0.29 |
| 8 |
Miller, Sarah E (11)
|
OHHURY |
38.04 |
39.21 |
1.17 |
| 9 |
Ganesh, Samiksha * (11)
|
OHLTPY |
39.64 |
39.77 |
0.13 |
| 10 |
Okonkwo, Constance E (12)
|
OHHURY |
42.63 |
39.89 |
-2.74 |
| 11 |
Heckman, Sophia M (12)
|
OHLTPY |
|
43.31 |
- |
| 12 |
Lingampally, Hamsini * (11)
|
OHLTPY |
50.83 |
47.24 |
-3.59 |
| 13 |
Wester, Carly A (11)
|
OHLTPY |
51.15 |
49.54 |
-1.61 |
|
Baker, Olivia A (12)
|
OHLTPY |
29.94 |
NS |
- |
|
Fogelman, Layla L (12)
|
OHLTPY |
31.38 |
NS |
- |
|
Legates, Ava S (12)
|
OHLTPY |
31.71 |
NS |
- |