| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fogelman, Layla L (12)
|
OHLTPY |
42.73 |
41.77 |
-0.96 |
| 2 |
Madsen, Natalie I (11)
|
OHLTPY |
45.52 |
42.20 |
-3.32 |
| 3 |
Reville, Maren A (11)
|
OHLTPY |
43.81 |
43.44 |
-0.37 |
| 4 |
Downey, Madeline P (12)
|
OHLTPY |
45.99 |
43.70 |
-2.29 |
| 5 |
Bowles, Audrey (12)
|
OHDRY |
50.42 |
43.97 |
-6.45 |
| 6 |
Brinkman, Madelyn M (11)
|
OHDRY |
44.10 |
44.11 |
0.01 |
| 7 |
Bair, Kate E (12)
|
OHLTPY |
42.43 |
45.03 |
2.60 |
| 8 |
Clark, Faith J (12)
|
OHDRY |
48.09 |
45.21 |
-2.88 |
| 9 |
Bettada, Neha V (11)
|
OHLTPY |
46.32 |
45.77 |
-0.55 |
| 10 |
Brown, Maggie R (11)
|
OHLTPY |
55.59 |
52.91 |
-2.68 |
| 11 |
Ganesh, Samiksha * (11)
|
OHLTPY |
1:07.40 |
1:06.22 |
-1.18 |
|
Bandhawakar, Saavni * (11)
|
OHLTPY |
1:01.38 |
DQ |
- |
|
Lingampally, Hamsini * (11)
|
OHLTPY |
|
DQ |
- |