| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ganger, Gracie N (13)
|
OHMCY |
37.63 |
38.33 |
0.70 |
| 2 |
Scolnick, Allison T (14)
|
OHGYG |
40.40 |
38.73 |
-1.67 |
| 3 |
Basham, Katelyn N (13)
|
OHHURY |
39.06 |
38.98 |
-0.08 |
| 4 |
Good, Julia K (13)
|
OHMCY |
39.33 |
39.43 |
0.10 |
| 5 |
Mechling, Skylar M (14)
|
OHLTPY |
38.85 |
39.90 |
1.05 |
| 6 |
Monhollen, Carley A (13)
|
OHEAST |
37.78 |
40.35 |
2.57 |
| 7 |
Stewart, Ellison J (13)
|
OHHURY |
41.02 |
40.66 |
-0.36 |
| 8 |
Harrison-Mills, Mya L (13)
|
OHLTPY |
46.45 |
40.96 |
-5.49 |
| 9 |
Brenner, Kerigan A (13)
|
OHMCY |
48.73 |
42.11 |
-6.62 |
| 10 |
D'Souza, Emily N (15)
|
OHLTPY |
41.35 |
43.69 |
2.34 |
| 11 |
D'Souza, Allie R (17)
|
OHLTPY |
43.29 |
46.26 |
2.97 |
| 12 |
Nollenberger, Molly A (13)
|
OHLTPY |
48.15 |
47.59 |
-0.56 |
| 13 |
Miller, Lillian M (13)
|
OHLTPY |
46.83 |
47.64 |
0.81 |
| 14 |
Valiquette, Megan (13)
|
OHCHY |
52.84 |
48.37 |
-4.47 |
| 15 |
Ahner, Alexis G (13)
|
OHHURY |
51.61 |
48.70 |
-2.91 |
| 16 |
Gudla, Sankeertana * (13)
|
OHLTPY |
55.00 |
54.92 |
-0.08 |