| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wooten, Hannah P (8)
|
NCRSA |
25.06 |
23.83 |
-1.23 |
| 2 |
Argenta, Brinkley E (8)
|
NCLTNC |
27.14 |
24.37 |
-2.77 |
| 3 |
Senko, Ruby Z (7)
|
NCRSA |
27.12 |
24.63 |
-2.49 |
| 4 |
Xin, Brianna H (8)
|
NCLTNC |
27.06 |
24.98 |
-2.08 |
| 5 |
Honnold, Emory C (7)
|
NCRSA |
28.52 |
25.90 |
-2.62 |
| 6 |
Eguiarte, Megan E (8)
|
NCLTNC |
|
25.94 |
- |
| 7 |
Martin, Annabelle E (8)
|
NCRSA |
39.33 |
28.49 |
-10.84 |
| 8 |
Strunk, Julia A (8)
|
NCRSA |
34.34 |
30.78 |
-3.56 |
| 9 |
Diem, Anya (7)
|
NCRSA |
55.17 |
35.42 |
-19.75 |
|
Santiago Raimundi, Gabriella (7)
|
NCUN |
|
26.08 |
- |
|
Minor, Anna E (7)
|
NCLTNC |
|
DQ |
- |
|
Powell, Alexis N (8)
|
NCRSA |
1:09.68 |
NS |
- |
|
Tischner, Kylen I (7)
|
NCRSA |
30.04 |
NS |
- |