| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Countie, Grace K (17)
|
NCMOR |
22.37 |
24.10 |
1.73 |
| 2 |
Clayton-Luce, Sophia H (17)
|
NCMOR |
23.63 |
25.52 |
1.89 |
| 3 |
Haney, Kenna L (16)
|
NCWAVE |
23.87 |
25.59 |
1.72 |
| 4 |
Francis, Sophia W (16)
|
NCMAC |
24.08 |
25.76 |
1.68 |
| 5 |
Jones, Morgan E (15)
|
NCSGSA |
24.51 |
25.81 |
1.30 |
| 6 |
Gaskey, Isabella S (15)
|
NCMAC |
24.78 |
25.94 |
1.16 |
| 7 |
Carlson, Alayna M (14)
|
NCMOR |
23.85 |
26.06 |
2.21 |
| 8 |
Steelhammer, Gracie B (16)
|
NCMAC |
25.19 |
26.18 |
0.99 |
| 8 |
Bruno, Hannah C (17)
|
NCMOR |
24.77 |
26.18 |
1.41 |
| 10 |
Barnett, Claudia J (17)
|
NCMAC |
25.34 |
26.21 |
0.87 |
| 11 |
Bridgham, Kayla L (16)
|
NCMOR |
25.74 |
26.28 |
0.54 |
| 12 |
Siemasko, Lily M (16)
|
NCMAC |
24.95 |
26.37 |
1.42 |
| 13 |
Whitaker, Peyton A (16)
|
NCMOR |
24.18 |
26.44 |
2.26 |
| 14 |
Mumford, Susan E (15)
|
NCMAC |
26.06 |
26.64 |
0.58 |
| 15 |
Fu, Jiayu (15)
|
NCWAVE |
24.76 |
26.80 |
2.04 |
| 16 |
Martinson, Elizabeth B (15)
|
NCMAC |
25.44 |
27.09 |
1.65 |
| 17 |
Scalise, Anna E (15)
|
NCMAC |
26.16 |
27.37 |
1.21 |
| 18 |
Blandford, Callie E (16)
|
NCMOR |
25.83 |
27.44 |
1.61 |
|
Ashley, Anna M (15)
|
NCMAC |
24.72 |
NS |
- |
|
Graham, Emerson L (17)
|
NCMAC |
25.65 |
NS |
- |
|
Snapper, Kierstin M (16)
|
NCMAC |
25.68 |
NS |
- |