| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tutovani, Levani D (16)
|
NCMAC |
|
52.36 |
- |
| 2 |
Sterrett, William J (15)
|
NCWOW |
|
53.07 |
- |
| 3 |
Moore, Rhett T (16)
|
SCCA |
|
53.52 |
- |
| 4 |
Cook, Alexander D (15)
|
NCMAC |
|
53.58 |
- |
| 5 |
Eckstein, Adam M (17)
|
MSSUNS |
|
56.41 |
- |
| 6 |
Phillipps, Maximus M (16)
|
NCMAC |
|
56.42 |
- |
| 7 |
Sanders, Sydney M (15)
|
NCMAC |
|
59.00 |
- |
| 8 |
Woodall, Katherine S (14)
|
KYLEXD |
|
59.24 |
- |
| 9 |
Hall, Lillian J (16)
|
SCTG |
|
59.46 |
- |
| 10 |
Albrecht, Sarah E (17)
|
KYLEXD |
|
1:00.53 |
- |
| 11 |
Yount, Carley M (16)
|
NCMAC |
|
1:01.51 |
- |
| 12 |
Perry, Audrey M (15)
|
SCH2A |
|
1:01.65 |
- |
| 13 |
Grasso, Aynslee M (17)
|
NCWAVE |
|
1:02.16 |
- |
| 14 |
Martinson, Ashley A (16)
|
NCMAC |
|
1:03.26 |
- |
|
McCarthy, Campbell P (17)
|
SCTG |
|
DQ |
- |