| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Elyse C (9)
|
NCMOR |
45.13 |
44.15 |
-0.98 |
| 2 |
DiGiovanni, Francesca E (10)
|
NCMOR |
49.13 |
47.88 |
-1.25 |
| 3 |
Kaidel, Kaya R (10)
|
NCMOR |
50.71 |
48.76 |
-1.95 |
| 4 |
Sauls, Macie A (10)
|
NCWAVE |
49.03 |
48.96 |
-0.07 |
| 5 |
Buff, Isabella R (10)
|
NCMOR |
50.00 |
49.36 |
-0.64 |
| 6 |
Zygmond, Jadyn B (10)
|
NCLTNC |
|
49.66 |
- |
| 7 |
Senko, Ruby Z (9)
|
NCRSA |
48.37 |
51.04 |
2.67 |
| 8 |
Schmidt, Lorelei E (9)
|
NCMOR |
48.58 |
51.16 |
2.58 |
| 9 |
Howell, Addison M (9)
|
NCRSA |
49.57 |
51.61 |
2.04 |
| 10 |
Strunk, Julia A (9)
|
NCRSA |
52.66 |
53.94 |
1.28 |
| 11 |
Felten, Evelyn A (9)
|
NCMOR |
1:30.93 |
53.95 |
-36.98 |
| 12 |
Irish, Natalie Y (9)
|
NCMOR |
58.99 |
54.89 |
-4.10 |
|
St.Pierre, Madaleine J (10)
|
NCRSA |
47.36 |
NS |
- |