| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jewell, Brynn H (16)
|
NCWOW |
29.11 |
29.14 |
0.03 |
| 2 |
Heineman, Ashley M (18)
|
NCWAVE |
28.34 |
29.57 |
1.23 |
| 3 |
Hoover, Madison M (15)
|
NCWOW |
31.15 |
30.40 |
-0.75 |
| 4 |
Edelson, Anna E (15)
|
NCWAVE |
29.74 |
30.42 |
0.68 |
| 5 |
Galloway, Claire E (15)
|
NCWAVE |
31.10 |
31.35 |
0.25 |
| 6 |
Fullerton, Mikaela C (16)
|
NCWAVE |
30.05 |
31.52 |
1.47 |
| 7 |
Jewell, Aleta M (18)
|
NCWOW |
29.76 |
32.27 |
2.51 |
| 8 |
Hurlbert, Katherine R (15)
|
NCWAVE |
31.36 |
32.31 |
0.95 |
| 9 |
McFadden, Kaleigh M (15)
|
NCSSS |
35.26 |
34.40 |
-0.86 |
| 10 |
Ball, Maureen E (15)
|
NCMOR |
35.67 |
34.68 |
-0.99 |
|
Denton, Emma G (15)
|
NCGFSC |
31.08 |
NS |
- |
|
Greene, Dana M (48)
|
NCWAVE |
42.18 |
NS |
- |
|
Henry, Elizabeth (16)
|
NCSSS |
28.53 |
NS |
- |
|
Johnson, Emma (15)
|
NCSSS |
34.34 |
NS |
- |
|
Chando, Terese E (16)
|
NCUNWA |
28.34 |
NS |
- |