| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Meyer, Penelope H (10)
|
ADDELM |
39.79 |
39.41 |
-0.38 |
| 2 |
Wolfort, Madison J (10)
|
ADALBS |
47.21 |
43.37 |
-3.84 |
| 3 |
Perri, Gabriella M (10)
|
ADDELM |
41.35 |
44.84 |
3.49 |
| 4 |
De Tuya, Elizabeth M (10)
|
ADDELM |
50.52 |
45.09 |
-5.43 |
| 5 |
Ziegler, Maggie S (10)
|
ADHAWK |
1:00.00 |
49.27 |
-10.73 |
| 6 |
Svare, Nuala S (10)
|
ADDELM |
1:05.18 |
52.41 |
-12.77 |
| 7 |
Cho, Anna Y (9)
|
ADALBS |
|
53.71 |
- |
| 8 |
Fargione, Brittany K (9)
|
ADDELM |
|
1:01.89 |
- |
| 9 |
Zhou, Olivia J (10)
|
ADALBS |
|
1:02.04 |
- |
| 10 |
Farrell, Macayla M (10)
|
ADALBS |
|
1:07.47 |
- |
| 11 |
Williams, Isabella R (10)
|
ADALBS |
|
1:19.72 |
- |
|
Ho, Sophie (10)
|
ADDELM |
|
DQ |
- |