| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brkanovic, Isak A (9)
|
ADDELM |
36.35 |
34.35 |
-2.00 |
| 2 |
Hopper, Andrew W (10)
|
ADDELM |
36.78 |
36.74 |
-0.04 |
| 3 |
Goodwill, Trevor M (10)
|
ADCPP |
37.07 |
36.92 |
-0.15 |
| 4 |
Rudd, Conner D (10)
|
ADCPP |
40.36 |
40.38 |
0.02 |
| 5 |
Killian, Daniel S (10)
|
ADCPP |
37.20 |
41.17 |
3.97 |
| 6 |
Cao, James Y (9)
|
ADDELM |
46.08 |
42.10 |
-3.98 |
| 7 |
Simmons, Cooper Z (10)
|
ADCPP |
42.75 |
45.36 |
2.61 |
| 8 |
Staudinger, Samuel P (9)
|
ADALBS |
47.14 |
47.06 |
-0.08 |
| 9 |
Leung, Arthur (10)
|
ADUN |
|
47.15 |
- |
| 10 |
LaFountain, Tobias P (10)
|
ADALBS |
|
1:03.31 |
- |
|
Zeliph, Thomas J (9)
|
ADALBS |
51.05 |
DQ |
- |
|
Hill, Nathaniel J (9)
|
ADCPP |
55.06 |
DQ |
- |
|
Upton, Matthew T (10)
|
ADCPP |
58.63 |
DQ |
- |
|
LaFountain, Jackson P (9)
|
ADALBS |
|
NS |
- |