| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huske, Victoria J (17)
|
PVAAC |
57.43 |
57.36 |
-0.07 |
| 2 |
Douglass, Katherine C (18)
|
VAUVA |
58.48 |
57.43 |
-1.05 |
| 3 |
Walsh, Alexandra J (19)
|
VAUVA |
51.77 |
59.58 |
7.81 |
| 4 |
Harter, Abigail E (18)
|
VAUVA |
59.62 |
59.83 |
0.21 |
| 5 |
Tankard, Kyleigh A (16)
|
VACGBD |
1:01.19 |
1:00.92 |
-0.27 |
| 6 |
Carr, Abby L (17)
|
PVRMSC |
1:01.04 |
1:01.26 |
0.22 |
| 7 |
McConagha, Mackenzie B (17)
|
PVNCAP |
1:01.19 |
1:01.61 |
0.42 |
| 8 |
Chong, Vanessa (17)
|
MRBAD |
1:01.13 |
1:01.62 |
0.49 |
| 9 |
Dahlke, Mikaela S (22)
|
PVMACH |
1:00.14 |
1:01.76 |
1.62 |
| 10 |
Cuomo, Alexa M (19)
|
VAUVA |
1:00.49 |
1:01.77 |
1.28 |
| 11 |
Jiang, Joy (17)
|
MRBAD |
1:01.81 |
1:01.82 |
0.01 |
| 12 |
Wall, Tatum E (16)
|
PVNCAP |
53.52 |
1:02.34 |
8.82 |
| 13 |
Runnels, Aris D (17)
|
PVNCAP |
1:02.40 |
1:02.52 |
0.12 |
| 14 |
Sebring, Carly A (16)
|
PVRMSC |
1:02.53 |
1:02.71 |
0.18 |
| 15 |
Witkus, Amanda E (15)
|
NEGS |
1:03.38 |
1:03.22 |
-0.16 |
| 16 |
Sheble, Caroline S (17)
|
VANOVA |
1:02.19 |
1:03.77 |
1.58 |