| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chapman, Jude A (12)
|
VAPSDN |
41.55 |
39.48 |
-2.07 |
| 2 |
Zhang, Jayden Y (11)
|
VANOVA |
41.37 |
40.75 |
-0.62 |
| 3 |
Hughes, Dylan J (11)
|
VANOVA |
41.48 |
40.97 |
-0.51 |
| 4 |
Lewis, David P (11)
|
VAPSDN |
45.17 |
41.39 |
-3.78 |
| 5 |
Ding, Brandon (12)
|
VANOVA |
45.14 |
42.67 |
-2.47 |
| 6 |
Lee, Joshua W (11)
|
VANOVA |
44.54 |
43.03 |
-1.51 |
| 7 |
Gardner, Jackson W (11)
|
VANOVA |
41.58 |
43.25 |
1.67 |
| 8 |
Chornodolsky, Bohdan A (12)
|
VAPSDN |
|
43.81 |
- |
| 9 |
Melson, Tyler C (11)
|
VANOVA |
45.64 |
44.25 |
-1.39 |
| 10 |
Cale, William G (11)
|
VANOVA |
47.62 |
45.29 |
-2.33 |
| 11 |
Danielson, Leo B (12)
|
VANOVA |
47.96 |
45.77 |
-2.19 |
| 12 |
Duque Lozada, Jesus M (11)
|
VANOVA |
|
47.37 |
- |
| 13 |
Holsten, Edward A (12)
|
VANOVA |
52.92 |
48.09 |
-4.83 |
| 14 |
Whitfield, Colton S (11)
|
VAPSDN |
54.85 |
52.58 |
-2.27 |
|
Almond, Donald K (11)
|
VANOVA |
|
DQ |
- |
|
Lehtinen, Calvin R (12)
|
VANOVA |
|
NS |
- |
|
Spears, Jacob O (11)
|
VANOVA |
|
NS |
- |