| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karasiewicz, Hayden A (7)
|
MDBAY |
20.22 |
19.14 |
-1.08 |
| 2 |
Stevens, Callie G (7)
|
VACGBD |
22.77 |
21.02 |
-1.75 |
| 3 |
Roitsch, Reagan E (8)
|
VACVAC |
23.35 |
24.37 |
1.02 |
| 4 |
Schwarzenberg-Word, Alia (8)
|
VACGBD |
25.09 |
24.60 |
-0.49 |
| 5 |
Goff, Emmalyn G (8)
|
VACGBD |
27.57 |
24.62 |
-2.95 |
| 6 |
Baker, Eliza M (8)
|
VASRVA |
26.44 |
26.26 |
-0.18 |
| 7 |
Raeburn, Morgan Delaney (7)
|
VACVAC |
27.38 |
26.85 |
-0.53 |
| 8 |
Pitts, Caitlin W (8)
|
MDBAY |
36.61 |
32.47 |
-4.14 |
| 9 |
Dexter, Abigail K (8)
|
MDBAY |
33.63 |
32.98 |
-0.65 |
| 10 |
Whiddon, Nina E (8)
|
VASRVA |
|
37.29 |
- |
| 11 |
Meledick, Hannah R (7)
|
MDBAY |
33.31 |
37.56 |
4.25 |
| 12 |
Quick, Leila K (8)
|
VACVAC |
|
38.07 |
- |
| 13 |
Shipe, Chloe E (8)
|
VACGBD |
|
42.89 |
- |
|
Lewis, Reagan Q (8)
|
MDBAY |
37.22 |
DQ |
- |
|
Richardson, Isabella J (8)
|
MDBAY |
|
DQ |
- |
|
Gonzalez, Ella G (6)
|
VACGBD |
57.96 |
DQ |
- |