| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schultz, Caroline L (14)
|
VA757 |
36.66 |
36.89 |
0.23 |
| 2 |
Pascal, Victoire (13)
|
VATIDE |
44.36 |
37.59 |
-6.77 |
| 3 |
Hall, Alayna C (14)
|
VA757 |
41.06 |
38.64 |
-2.42 |
| 4 |
Muscolino, Aubrey S (13)
|
VATIDE |
42.49 |
39.16 |
-3.33 |
| 5 |
Berg, Chloe M (13)
|
VATIDE |
41.20 |
39.42 |
-1.78 |
| 6 |
Moose, Kayla M (14)
|
VAWAC |
|
39.82 |
- |
| 7 |
Shields, Sophia A (14)
|
VATIDE |
46.72 |
40.51 |
-6.21 |
| 8 |
Raeburn, Quinn C (13)
|
VATIDE |
41.07 |
40.96 |
-0.11 |
| 9 |
Hoadley, Addison G (13)
|
VA757 |
49.41 |
43.94 |
-5.47 |
| 10 |
Kirkpatrick, Caeleigh A (13)
|
VATIDE |
46.36 |
44.04 |
-2.32 |
| 11 |
Hoy, Addison S (13)
|
VAUN-T |
|
45.40 |
- |
| 12 |
Kirkpatrick, Anna R (13)
|
VATIDE |
54.14 |
49.32 |
-4.82 |
| 13 |
Hayenga, Natalia N (14)
|
VATIDE |
51.47 |
50.05 |
-1.42 |
| 14 |
Mateo-Henao, Laura M (13)
|
VATIDE |
59.52 |
58.49 |
-1.03 |
|
Weisiger, Ali K (13)
|
VA757 |
|
NS |
- |